Tuesday, April 25, 2017

Formocresol Mutagenicity following primary tooth pulp therapy: an in vivo study

Department of Pediatric Dentistry
Lutheran Medical Center

Resident’s Name: Nicholas Paquin                                                                           Date: 04/26/17

Article Title: Formocresol Mutagenicity following primary tooth pulp therapy: an in vivo study
Author(s): P.A. Zarzar, Rosenblatt, Takashashi, Takeuchi, Costa Junior
Journal:  Journal of Dentistry
Date:  April 2003
Major Topic: Formocresol
Type of Article: Case-Control study
Main Purpose: Determine the mutagenicity of formocresol
Key Points/Summary:  This study was designed to look at the mutagenicity of full strength Buckley’s formocresol on in vivo lymphocytes cultures obtained from the peripheral blood of children, ages 5-10, who had one pulpotomy completed in the clinics of Recife City Council and the University of Pernambuco School of Dentistry, Brazil

Methods:  A blood sample from 20 children was collected prior to the pulpotomy (control group) and 24 after treatment was completed with Buckley’s full strength formocresol.  The peripheral lymphocytes were grown in a complete culture medium and assessed for chromosomal aberrations via a previously published method that was modified.  The cytogenetic analysis was performed in a blind test.

Results:  There was no statistically significant difference between the groups using Wilcoxon’s Signed Ranks test for chromosomal aberrations and for the total chromosomal breaks.  However the solution was mutagenic for one patient, raising concern for use as a pulp treatment.

Conclusion: Results revealed that, from a statistical standpoint, formocresol is not mutagenic.  However, further investigations are required, preferably with a larger sample, in patients needing more than one pulpotomy to determine if an increase in the quantity of the drug would increase the quantity of the chromosomes aberrations. 

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